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. 2018 Aug 9;8:11905. doi: 10.1038/s41598-018-30309-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of LH2 or LH3 promotes lung cancer cell growth and migration. (A–D) Western blotting (A), soft agar colony formation, (B), migration (C), and invasion (D) assays for 393 P cells stably expressing FLAG-tagged LH2 and LH3 or empty vectors. (E) Microscopic images for the GATA3-depleted 344SQ cells that stably express vector or FLAG-tagged LH2 and migrated through non-coated transwells. (F) The number of migrated cells were counted and expressed as mean + stdv. (G) Microscopic images for the HCC827 cells that stably express vector or FLAG-tagged LH3 and migrated through non-coated transwells. (H) The number of migrated cells were counted and expressed as mean + stdv. (I–K) MTT (I), migration (J), and invasion (K) assay for 393 P cells transfected with scrambled (scr) or GATA3 shRNAs. Note: in all figures, *indicates t-test p < 0.05.