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. 2018 Apr 26;32(8):1359–1364. doi: 10.1038/s41433-018-0084-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Expression of WT-MIP and K228fs-MIP in cultured HeLa cells. a The mRNA transcription level of K228fs-MIP was decreased compared with that of WT-MIP according to quantitative real-time PCR (T-TEST, two-sided, *P = 0.0052, error bars represent standard deviation, SD). All of the samples were analyzed in four replicates and normalized to median β-actin expression. b Western blots were performed as indicated and showed that the protein expression of K228fs-MIP was lower than that of WT-MIP. The expression of β-actin was used as the control. c Subcellular localization of HA-WT-MIP and HA-K228fs-MIP were determined after transient transfection in cultured HeLa cells. Confocal laser scanning images showed the distribution of HA-tagged MIP (red) and DAPI-stained nuclei (blue). HA-WT-MIP was detected mainly in the plasma membrane with less observed in the cytoplasm, but K228fs-MIP was largely localized in the cytoplasm. Scale bar: 5 μm