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. 2018 Aug 9;8:11906. doi: 10.1038/s41598-018-30494-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative scanning electron microscopy of in vivo MRSA biofilm (A) Isolate 1 showing an in vivo detached biofilm at low magnification. Sometimes the sample processing for scanning electron microscopy released the biofilm cluster from the endotracheal tube surface. At higher magnification (B), cocci morphologies can be distinguished. The pig from which we obtained Isolate 1 was treated with vancomycin. (C) Isolate 45 (from a placebo treated pig) showing an in vivo biofilm attached to the endotracheal tube at low magnification. (D) at higher magnification, a cocci biofilm cluster was found (white arrow). Abbreviations: MRSA, methicillin-resistant Staphylococcus aureus.