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. 2018 Aug 9;8:11906. doi: 10.1038/s41598-018-30494-7

Figure 5.

Figure 5

Representative confocal laser scanning microscopy of in vivo MRSA biofilm Biofilm clusters (white arrows) were stained with the LIVE/DEAD BacLight kit (INVITROGEN, Barcelona, Spain). Viable bacteria (stained green by SYTO 9) are visible, but dead bacteria (stained red by propidium iodide) were infrequently detected. The nuclei and cytoplasm of eukaryotic cells from the pig were also stained nonspecifically by propidium iodide and SYTO 9 (large red and green blotches, respectively). (A,B) correspond to the in vivo biofilms of Isolate 39 and Isolate 45 obtained from pigs treated with vancomycin and placebo, respectively. Abbreviations: MRSA, methicillin-resistant Staphylococcus aureus.