Fig. 1.

Biosynthetic steps catalyzed by LmbA/Ant6 and LmbW/Ant5 in the context of ALDP pathway. a Scheme of ALDP biosynthetic pathway (adopted from Jiraskova et al.² and modified according to Kamenik et al.¹⁰); Dotted arrows indicate steps proposed by Zhong et al.¹, brackets indicate a side-pathway, final ALDP precursors highlighted in blue are incorporated into the secondary metabolites. b In vitro (experiments from Jiraskova et al.² re-examined using a more suitable chromatographic method) and in vivo (new experiments) C-methylation of 2/3 by LmbW; Chromatographic conditions: UPLC BEH Amide 1.7 µm, 2.1 × 50 mm column (Waters, USA), mobile phase: A-acetonitrile and B-50 mM ammonium acetate pH8:acetonitrile 1:1 (v/v), elution: 99% A for 2.5 min followed by a linear decrease from 99 to 1% A in 10 min, UV/VIS chromatograms extracted at 405 nm, MS spectra were recorded using an electrospray ionization technique in a negative mode