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. 2018 Aug 9;8:11935. doi: 10.1038/s41598-018-29817-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nuclear biomechanical analysis reveal stiffer nuclei in PGCCs. Ensemble averaged MSDs derived from chromatin granule motion within nuclei of (A) non-PGCCs and (B) PGCCs show decreased particle motion and increased nuclear stiffness in PGCCs (100 + traces, 30 + cells). (C) Coefficient of variation analysis for ensemble MSDs of non-PGCC and PGCCs reveal increased heterogeneity in nuclear mechanics for PGCCs. (D) Fluorescent images of non-PGCC and PGCCs (white arrow) stained with anti-lamin A/C (nuclear lamin, green) and DAPI (nucleus, blue). (E) Fluorescence intensity quantification of lamin A/C expression in MDA cells reveal no significant differences between PGCCs and non-PGCCs. (F) Quantification of heterochromatic foci density show increased number of foci as a function of area in PGCCs. Results are reported as the mean ± SEM. Significance is indicated as *p < 0.05, **p < 0.01, ***p < 0.001.