Figure 5.

Adoptive transfer of cannabidiol (CBD)-induced myeloid-derived suppressor cells (MDSCs) attenuates experimental autoimmune encephalomyelitis (EAE). (A) Time line schematic of studies. CBD-induced MDSCs were administered at time points with gray arrows (d7 and d9) via i.p. injection. Data were assessed at peak of disease unless otherwise stated. CBD-induced MDSCs from IP cavity were collected and 4 × 10⁶ MDSCs or splenocytes from naïve C57BL/6 as a control were adoptively transferred. (B) Clinical scores (n = 5 mice per group); data were presented as mean ± SEM and analyzed for significance using Mann–Whitney U test. Comparisons were considered significant at p ≤ 0.05, denoted as *. Data are representative of at least two independent experiments; in each experiment, disease incidence was 100% for each group. (C) Total mononuclear cell infiltrates in central nervous system and absolute cell counts for CD3⁺CD4⁺ and CD3⁺CD8⁺ T cells; mononuclear cells stained with corresponding Abs and then enumerated using total cell count and frequency from flow cytometry. (D) Mononuclear cells stained with CD11b and Gr1 Abs and then analyzed by flow cytometry. Representative histograms shown. (E) Absolute cell counts for MDSC (CD11b⁺Gr1⁺) enumerated using total cell count and frequency from flow cytometry. (F) Splenocytes were stained with CD11b and Gr1 Ab and then analyzed by flow cytometry. Representative histograms shown. Vertical bars in this figure represent data collected from three to five mice per group expressed as mean ± SEM. ANOVA, ***p < 0.0001, and *p < 0.05 with Tukey’s post hoc test.