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. 2018 Aug 3;12:217. doi: 10.3389/fncel.2018.00217

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Copyright © 2018 Hlushchenko, Khanal, Abouelezz, Paavilainen and Hotulainen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibitory synapse localization in a DIV15 hippocampal neuron expressing mCherry and stained with postsynaptic anti-gephyrin (Alexa-488) and presynaptic anti-VGAT (Alexa-594) antibodies. In overlay, mCherry is shown in red, anti-gephyrin antibody staining in green, and anti-VGAT antibody staining in magenta. The line indicates the contour of dendrites (from mCherry). Scale bar, 5 μm.