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. 2018 Aug 3;12:217. doi: 10.3389/fncel.2018.00217

Figure 7.

Figure 7

Characterization of the inhibitory synapses in DIV15 hippocampal neurons expressing either mCherry (control), wild-type myosin IIb-mCherry or myosin IIb-Y265C-mCherry and stained with anti-gephyrin antibody. (A) In overlay, mCherry is shown in magenta and anti-gephyrin antibody staining in green. The line indicates the contour of dendrites (from mCherry). Scale bars, 5 μm. (B) Quantification of inhibitory synapse properties. From left to right: (1) Mean density of synapses calculated as the number of gephyrin puncta per μm of the dendrite: control = 0.37; myosin IIb = 0.45; myosin IIb-Y265C = 0.39. (2) Mean diameter of individual gephyrin puncta in μm: control = 0.44; myosin IIb = 0.46; myosin IIb-Y265C = 0.42 3. Proportion of the gephyrin puncta located on dendritic spines as a fraction of the total number of puncta: control = 0.33; myosin IIb = 0.43; myosin IIb-Y265C = 0.34. Data in (B) represent n(control) = 17 cells, n(myosin IIb) = 20 cells, n(myosin IIb-Y265C) = 19 cells, pooled from 3 experiments. *p < 0.05 and **p < 0.01 as determined by one-way ANOVA with Bonferroni's post-hoc test. Data is represented as mean ±SEM.