Table 4.
A total of 210 A. cerana larvae were randomly selected to detect CSBV by RT-PCR.
| Geographic location | Classification of colony infected | Number of samples | Ratio of positive to negative samples at different time points from 2 to 8 days (a/b) |
||||||
|---|---|---|---|---|---|---|---|---|---|
| 2 | 3 | 4 | 5 | 6 | 7 | 8 | |||
| Huairen | Serious infection | 35 | 5/0 | 5/0 | 5/0 | 5/0 | 2/3 | 0/5 | 0/5 |
| Moderate infection | 35 | 5/0 | 1/4 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | |
| Kuandian | Serious infection | 35 | 5/0 | 5/0 | 2/3 | 1/4 | 0/5 | 0/5 | 0/5 |
| Moderate infection | 35 | 5/0 | 2/3 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | |
| Chengde | Serious infection | 35 | 5/0 | 5/0 | 5/0 | 3/2 | 1/4 | 0/5 | 0/5 |
| Moderate infection | 35 | 5/0 | 1/4 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | |
a and b represent the number of positive and negative samples for detecting CSBV by RT-PCR, respectively. Number of sample 35 means that 5 A. cerana larvae were randomly selected on 2, 3, 4, 5, 6, 7, and 8 days after treatment.