FIGURE 5.

Membrane permeabilization of CCCP- induced P. aeruginosa persister cells using propidium iodide (PI) through spectrophotometry. Permeabilization of persister cells formed in stationary phase cultures of P. aeruginosa cultures were treated with ciprofloxacin (100 μg/mL), tobramycin (16 μg/mL), colistin (256 μg/mL), azithromycin (256 μg/mL), aztreonam (256 μg/mL), and clarithromycin (256 μg/mL) or PAAG (100–200 μg/mL) and measured by spectrophotometry over 4 h. PI fluorescence was measured pre- and post-treatment with antibiotics or PAAG. CCCP-induced cells with PI was used as a baseline control. The experiment was repeated with there independent cultures and the data is represented as the average ±standard error of the mean.