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. 2018 Aug 3;9:1724. doi: 10.3389/fmicb.2018.01724

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Copyright © 2018 Narayanaswamy, Keagy, Duris, Wiesmann, Loughran, Townsend and Baker.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PAAG upon permeabilization causes depolarization of the cytoplasmic membrane. Effect of PAAG or colistin on the cytoplasmic membrane of CCCP-induced P. aeruginosa persister cells incubated with DiSC3(5). Results expressed in relative fluorescence units (RFU) observed at 670 nm as compared to negative control. 0.1% Triton X-100 was used as positive control. Cells with DiSC3(5) was used as the baseline control. Data represented as mean ± SEM of three determinations. ^∗∗∗∗P < 0.001, ^∗∗P < 0.01, ^∗P < 0.05. The lines were used to compare the statistical significance between the different concentration of PAAG treatments.