Table 1.

Substrate specificities of D-AADH and its mutants.

Substrate	Oxidative deamination
	His-tagged D-AADH Specific activity (μmol/min/mg)	Non-tagged D-AADH Specific activity (μmol/min/mg)	Asp94Ala Specific activity (μmol/min/mg)	Tyr224Phe Specific activity (μmol/min/mg)
D-Arginine	0.00738 ± 0.0002	0.722 ± 0.024	0.0815 ± 0.0027	0.337 ± 0.012
D-Histidine	-	0.0168 ± 0.0003	0.139 ± 0.003	0.0139 ± 0.0001
D-Isoleucine	0.193 ± 0.003	0.113 ± 0.002	1.70 ± 0.05	0.0763 ± 0.0016
D-Leucine	0.106 ± 0.004	0.191 ± 0.002	4.80 ± 0.11	0.0895 ± 0.0015
D-Lysine	0.299 ± 0.001	10.8 ± 0.1	1.32 ± 0.06	2.25 ± 0.02
D-Methionine	0.0178 ± 0.0009	0.213 ± 0.006	2.60 ± 0.11	0.173 ± 0.004
D-Norleucine	0.107 ± 0.002	0.419 ± 0.020	4.22 ± 0.10	0.315 ± 0.008
D-Phenylalanine	0.0106 ± 0.0002	0.099 ± 0.002	5.33 ± 0.14	0.0769 ± 0.0005
D-Tryptophan	-	0.0369 ± 0.0020	0.244 ± 0.006	0.0186 ± 0.0002
D-Valine	0.0142 ± 0.0008	0.121 ± 0.004	0.185 ± 0.005	0.0711 ± 0.0017
	Reductive amination
2-Oxooctanoate	2.27 ± 0.06	13.2 ± 0.1	65.5 ± 0.8	11.7 ± 0.5
Phenylpyruvate	0.235 ± 0.005	1.93 ± 0.02	16.1 ± 0.3	1.67 ± 0.02
Pyruvate	0.0688 ± 0.0020	0.470 ± 0.029	0.131 ± 0.004	0.138 ± 0.003
2-Oxobutanoate	0.201 ± 0.002	2.47 ± 0.03	0.859 ± 0.052	0.85 ± 0.01
2-Oxohexanoate	1.03 ± 0.06	9.21 ± 0.13	35.0 ± 0.9	7.56 ± 0.17
2-Oxopentanoate	0.465 ± 0.015	4.72 ± 0.08	7.63 ± 0.20	3.44 ± 0.10
2-Oxo-3-methylbutanoate	0.279 ± 0.004	2.54 ± 0.05	1.04 ± 0.03	1.89 ± 0.01
2-Oxo-3-methylpentanoate	0.310 ± 0.007	2.23 ± 0.05	3.90 ± 0.06	2.25 ± 0.07
2-Oxo-4-methylpentanoate	0.624 ± 0.012	4.78 ± 0.11	15.7 ± 0.2	4.64 ± 0.06
2-Oxo-4-methylthio butanoate	0.624 ± 0.012	6.37 ± 0.09	25.0 ± 0.8	6.22 ± 0.12