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Golgin-97 Interacts with Kir2.1 in the Golgi. (A) Confocal images of Kir2.1 (TGN-export mutant (Δ314–315) compared to ER-export mutant ΔFCY) in COS-cells (green) and Golgi-97 (red) before (top) and after 10 min of brefeldin A treatment. (B) Anti-Golgin-97 immunoprecipitation (IP) followed by anti-HA immunoblotting (IB) from COS cells transfected with the HA-Kir2.1 mutants and treated with vehicle (–) or Brefeldin (+).
