Table 2.
Carbohydrate transport and utilization genes that are highly expressed in MGGal+ grown in CDM-gal as compared to CDM-fru – grown MGGal+.
| Locus | Gene | Growth phase I | Growth phase II | Function |
|---|---|---|---|---|
| llmg_2234 | galT | 76.9 | 18.6 | Galactose 1-P uridyltransferase |
| llmg_2235 | galK | 71.6 | 38.3 | Galactokinase |
| llmg_2236 | galM | 55.8 | 28.9 | Galactose mutarotase |
| llmg_2237 | galP | 44.2 | 24.2 | Galactose permease |
| llmg_2233 | galE | 11.0 | 4.0 | UDP-galactose-4-epimerase |
| llmg_0438 | ptcA | 57.9 | 50.7 | Glucose/cellobiose PTS IIA component |
| llmg_0439 | llmg_0439 | 33.1 | 17.1 | LacI family transcriptional regulator |
| llmg_0440 | ptcC | 4.4 | 4.8 | Glucose/cellobiose PTS IIC component |
| llmg_0437 | ptcB | NA | NA | Glucose/cellobiose PTS IIB component |
| llmg_0727 | ptnD | 11.8 | 1.9 | Glucose/mannose PTS IID component |
| llmg_0728 | ptnC | 3.3 | 2.1 | Glucose/mannose PTS IIC component |
| llmg_0729 | ptnAB | 2.8 | 2.8 | Glucose/mannose PTS IIAB component |
| llmg_pseudo54 | llmg_pseudo54 | 5.8 | 5.0 | Pseudogene IIC PTS component |
| llmg_0739 | malE | 16.5 | 13.1 | Maltose ABC transporter substrate binding protein |
| llmg_0738 | malF | 11.0 | 9.6 | Maltose ABC transporter |
| llmg_0737 | malG | 4.6 | 5.5 | Maltose ABC transporter permease |
| llmg_0746 | malR | 2.1 | 2.3 | Maltose operon transcriptional repressor |
| llmg_0446 | msmK | 22.3 | 16.1 | Multiple sugar-binding transport ATP-binding protein |
| llmg_1569 | fruC | –4.5 | 1.1 | Tagatose-6-phosphate kinase |
| llmg_1568 | fruA | –3.8 | –1.3 | PTS system, fructose specific IIBC components |
| llmg_1570 | fruR | –3.1 | 1.3 | Fructose operon transcriptional repressor |
Column “Growth phase I” shows fold upregulation of genes in cells harvested during early exponential phase (OD600 of 0.1); column “Growth phase II” – that of cells grown till late exponential phase (OD600 of 0.35). Only significantly expressed genes are included (Bayesian P < 0.001); “NA” specifies that the gene was not significantly expressed. The numbers between the two columns are not directly comparable.