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High-performance liquid chromatography (HPLC) gradient program used to separate phytohormones.
| Time (min) | Methanol (%) | 0.01% Formic acid (%) | Flow rate (mL/min) |
|---|---|---|---|
| 10 | 5.0 | 95.0 | 0.15 |
| 40 | 15.0 | 85.0 | 0.15 |
| 50 | 85.0 | 15.0 | 0.15 |
| 60 | 5.0 | 95.0 | 0.15 |
| 70 | 5.0 | 95.0 | 0.15 |
