FIGURE 3.

Tp53 mutation may promote WISP1 expression in SH-PAN and PDAC cells. After lentivirus-mediated mouse Tp53^R172H overexpression vector or control was stably transduced into SH-PAN cells, lentivirus-mediated human Tp53^R175H or Tp53^R273H overexpression vector or control was stably transduced into Capan-2 cells (Tp53 wild-type), lentivirus-mediated human wild-type Tp53 overexpression vector or control was stably transduced into Panc-1 cells (Tp53 mutation), and then immunofluorescence staining was performed to measure the levels and locations of WISP1 in SH-PAN (A,B), Capan-2 (C,D), and Panc-1 cells (E,F) (white bar scale = 10 μm).