Table 7.
Genetic analysis using SNP and SSR nuclear markers located on LGs 7, 8, and 9 and mitochondrial (mt) and chloroplastic (cp) markers performed on SMC-58 and SMC-73 somatic hybrids that were obtained by protoplast fusion between C. macrophylla (CM) and Carrizo citrange (CC).
| Locus | LG | CC | CM | SMC-58 | SMC-73 | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| mCrCIR07E05 | 7 | z119 | 128 | 116 | 119 | 128 | 116 | 119 | 128 | 116 | |||
| DXS-M618 | yG | G | A | A | G | G | A | A | G | G | A | A | |
| DXS-C545 | G | G | C | G | G | G | C | G | G | G | C | G | |
| FLS-P129 | C | T | T | T | C | T | T | T | C | T | T | T | |
| Ci07C07 | 212 | 239 | 212 | 239 | 212 | 239 | |||||||
| mCrCIR07B05 | 8 | 196 | 203 | 210 | 196 | 203 | 210 | 196 | 203 | 210 | |||
| CiBE0214 | 312 | 309 | 312 | 309 | 312 | 309 | |||||||
| CiC5164-02 | C | C | T | T | C | C | T | T | C | C | T | T | |
| CiC1749-05 | G | T | T | T | G | T | T | T | G | T | T | T | |
| mCrCIR07F11 | 9 | 160 | 162 | 164 | 160 | 162 | 164 | 160 | 162 | 164 | |||
| CiC2518-02 | T | A | T | T | T | A | T | T | T | A | T | T | |
| Ci08C05 | 153 | 153 | 156 | 153 | 153 | 156 | 153 | 153 | 156 | ||||
| LCYB-M480 | T | C | T | T | T | C | T | T | T | C | T | T | |
| HYB-M62 | A | A | C | C | A | A | C | C | A | A | C | C | |
| HYB-C433 | G | G | A | G | G | G | A | G | G | G | A | G | |
| nad2/4-3 | mt | 261 | 251 | 251 | 251 | ||||||||
| CCMP2 | cp | 197 | 203 | 197 | 203 | 197 | |||||||
| CCMP5 | cp | 93 | 95 | 93 | 95 | 93 | |||||||
| CCMP6 | cp | 133 | 135 | 133 | 135 | 133 | |||||||
| NTCP7 | cp | 182 | 188 | 182 | 188 | 182 | |||||||
y
SNP alleles: A, adenine; C, cytosine; T, thymine; G, guanine.
z
SSR allele: numbers are the allele size in nucleotides. Lost SSR alleles and modified SNP alleles are marked in gray.