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Cytokine production by unfractionated MLN cells. Pooled MLN cells from each group were incubated in the presence of 20 μg/ml of SEA. IL-2 in culture supernatants was measured at 24 h; the other cytokines were measured at 48 h. Data are expressed as in Fig. 3. IFN-γ and IL-2 levels were significantly higher (P < 0.01), whereas IL-4, IL-5, IL-10, and IL-13 levels were significantly lower (P < 0.05) in SEA/CFA-immunized mice than in all BL/6 controls.
