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Cytokine production by purified CD4 T cells from MLNs. Pooled CD4 T cells from MLNs were cultured with irradiated normal splenic antigen-presenting cells and 20 μg/ml of SEA. IFN-γ and IL-5 were measured in 48-h culture supernatants. Data are expressed as in Fig. 3. IFN-γ levels were significantly higher and IL-5 levels were significantly lower in SEA/CFA-immunized mice than in the controls (both P < 0.01).
