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Cytokine production by unfractionated spleen cells. Pooled spleen cells from each group were incubated in the presence of 20 μg/ml of SEA. IL-2 in culture supernatants was measured at 24 h; the other cytokines were measured at 48 h. Data are expressed as in Fig. 3. IFN-γ and IL-2 levels were significantly higher, and IL-5 and IL-10 levels were significantly lower in SEA/CFA-immunized mice than in all BL/6 controls (both P < 0.01).
