Fig. 2.

Lung epithelial barrier is severely injured during H7N9-induced lung inflammation. (A–B) The biopsied tissues isolated from patient 2 are analyzed using H&E staining of normal lung tissue adjacent to the lung adenocarcinoma (Normal) as the control. (C) Electron microscopic scanning identifies the formation of autophagosomes in alveolar type 2 cells from patient 2 (arrows). Lamellar bodies in alveolar type 2 cells are indicated using arrow heads. (D–G) Expression of claudins and alveolar surfactants is analyzed in the biopsied lung tissue by using quantitative PCR. Relative levels to β-actin and fold changes (H7N9/Normal) are presented separately. (H–K) Expression of airway mucins and claudins is analyzed and presented in the same manner as above.