Fig. 5.

Differentially rhythmic expression patterns of splicing-related genes in CRC progression include amplitude changes and phase shifts.

Selected circadian transcriptional expression patterns in SW480 (blue line) and SW620 cells (green line) of spliceosome components and SFs that showed higher amplitudes in SW480 cells (blue box), higher amplitudes in SW620 cells (green box), as well as phase-shifted oscillations that persisted in both CRC cell lines (pink box). Transcripts that were identified as circadian are represented by fitted harmonic regression curves. The log₂ FC was calculated as the change of the mean expression over all time points from SW480 to SW620 cells. The adjusted p-value denotes the results of the DODR analysis and was calculated as previously described.