Fig. 6.

Differential AS events in SW480 and SW620 cells are associated with proliferation, angiogenesis, and cell adhesion.

(a) Paired differences in FIRMA scores of the top 30 candidate exons with differential AS events in SW480 and SW620 cells (BH adjusted limma p < 0·001 and absolute FIRMA score log₂ FC ≥ 1·35). The color scale is not evenly spaced but based on the percentiles of all FIRMA scores for all probesets and samples after filtering. The respective genes are annotated with GO terms that were found to be enriched in (b). (b) Selected enriched GO terms (biological processes) for the 256 candidate genes with differential AS events (BH adjusted limma p < .001 and absolute FIRMA score log₂ FC ≥ 1). (c) Exon-level expression (upper panel), FIRMA scores (middle panel), and genomic representation (lower panel) of the candidate gene NCAM1. Vertical lines separate the individual probesets covering the gene. For each probeset, the exon-level expression and the FIRMA scores of the individual time points are depicted by a dotted line and the respective mean values are depicted by a solid line for SW480 (blue) and SW620 (green), respectively. The mean FIRMA score log₂ FC (SW480-SW620) is shown in pink. Gray diagonal lines indicate the localization of the probesets within the genome.