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. 2018 Apr 16;6(2):215–217.e8. doi: 10.1016/j.jcmgh.2018.04.005

Supplementary Figure 1.

Supplementary Figure 1

Optimization of iron loading in SGBS cells. (A) Iron assay, P = .004 (1-way analysis of variance [ANOVA]), *P < .01 (Dunnett multiple comparisons test compared with 0 μmol/L FAC, n = 2 per group). (B) Total RNA (P = NS by 1-way ANOVA, N = 3 per group). (C) Total lysate protein (P = NS by 1-way ANOVA, n = 3 per group). (D) Total secretome protein (P = NS by 1-way ANOVA, N = 3 per group). (E) Viability assay (MTS) (P = NS by 1-way ANOVA, N = 3 per group). Data are presented as means and SEM. MTS, [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium].