Supplementary Table 1.
List of SGBS Secretome Proteins With Significantly Altered Signal Intensity in Response to Iron
| Accession number | Gene name | Protein name | Mean signal intensity ratio, iron/vehicle | SD | P value |
|---|---|---|---|---|---|
| Q8IX30 | SCUBE3 | Signal peptide, CUB and EGF-like domain-containing protein 3 | 0.026 | 0.016 | .001 |
| P61353 | RPL27 | 60S ribosomal protein L27 | 0.060 | 0.005 | .001 |
| Q9NQH7 | XPNPEP3 | Probable Xaa-Pro aminopeptidase 3 | 0.076 | 0.004 | .001 |
| P07996 | THBS1 | Thrombospondin-1 | 0.083 | 0.047 | .001 |
| Q76M96 | CCDC80 | Coiled-coil domain-containing protein 80 | 0.090 | 0.031 | .001 |
| P78539 | SRPX | Sushi repeat-containing protein SRPX | 0.096 | 0.620 | .001 |
| Q9UHI8 | ADAMTS1 | A disintegrin and metalloproteinase with thrombospondin motifs 1 | 0.114 | 0.090 | .002 |
| Q92538 | GBF1 | Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 | 0.118 | 0.024 | .004 |
| Q15063 | POSTN | Periostin | 0.121 | 0.149 | .001 |
| P08238 | HSP90AB1 | Heat shock protein (HSP) 90-β | 0.125 | 0.038 | .004 |
| P24593 | IGFBP5 | Insulin-like growth factor-binding protein 5 | 0.154 | 0.069 | .001 |
| Q15113 | PCOLCE | Procollagen C-endopeptidase enhancer 1 | 0.155 | 0.088 | .001 |
| Q9NTX5 | ECHDC1 | Ethylmalonyl-CoA decarboxylase | 0.158 | 0.068 | .001 |
| P25788 | PSMA3 | Proteasome subunit α type 3 | 0.176 | 0.442 | .001 |
| Q12931 | TRAP1 | Heat shock protein 75 kilodaltons, mitochondrial | 0.186 | 0.028 | .018 |
| P04083 | ANXA1 | Annexin A1 | 0.192 | 0.049 | .001 |
| P30101 | PDIA3 | Protein disulfide-isomerase A3 | 0.198 | 0.059 | .001 |
| Q99985 | SEMA3C | Semaphorin-3C | 0.199 | 0.225 | .001 |
| Q6NZI2 | PTRF | Polymerase I and transcript release factor | 0.210 | 0.134 | .001 |
| Q8TAV4 | STOML3 | Stomatin-like protein 3 | 0.220 | 0.084 | .006 |
| Q9UKZ9 | PCOLCE2 | Procollagen C-endopeptidase enhancer 2 | 0.226 | 0.030 | .001 |
| Q05469 | LIPE | Hormone-sensitive lipase | 0.231 | 0.196 | .023 |
| Q13642 | FHL1 | Four and a half LIM domains protein 1 | 0.263 | 0.006 | .029 |
| P02749 | APOH | β2-glycoprotein 1 | 0.286 | 0.310 | .048 |
| P02462 | COL4A1 | Collagen α-1(IV) chain | 0.286 | 0.351 | .001 |
| P15311 | EZR | Ezrin | 0.296 | 0.077 | .003 |
| P42765 | ACAA2 | 3-Ketoacyl-CoA thiolase, mitochondrial | 0.308 | 0.136 | .001 |
| P68104 | EEF1A1 | Elongation factor 1-α 1 | 0.309 | 0.215 | .001 |
| Q9NQC3 | RTN4 | Reticulon-4 | 0.312 | 0.119 | .007 |
| Q8IY17 | PNPLA6 | Neuropathy target esterase | 0.316 | 0.028 | .026 |
| Q92743 | HTRA1 | Serine protease HTRA1 | 0.324 | 0.075 | .001 |
| P08294 | SOD3 | Extracellular superoxide dismutase (Cu-Zn) | 0.339 | 0.027 | .005 |
| Q16836 | HADH | Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial | 0.348 | 0.207 | .011 |
| Q99715 | COL12A1 | Collagen α-1(XII) chain | 0.348 | 0.183 | .001 |
| P07355 | ANXA2 | Annexin A2 | 0.354 | 0.111 | .001 |
| P26038 | MSN | Moesin | 0.362 | 0.110 | .034 |
| P23284 | PPIB | Peptidyl-prolyl cis-trans isomerase B | 0.374 | 0.122 | .001 |
| O94769 | ECM2 | Extracellular matrix protein 2 | 0.375 | 0.060 | .005 |
| Q9NRN5 | OLFML3 | Olfactomedin-like protein 3 | 0.389 | 0.066 | .002 |
| P53396 | ACLY | Adenosine triphosphate–citrate synthase | 0.389 | 0.066 | .001 |
| Q16363 | LAMA4 | Laminin subunit α-4 | 0.407 | 0.091 | .001 |
| P14625 | HSP90B1 | Endoplasmin | 0.414 | 0.068 | .025 |
| Q9BU40 | CHRDL1 | Chordin-like protein 1 | 0.419 | 0.264 | .001 |
| P02649 | APOE | Apolipoprotein E | 0.421 | 0.053 | .001 |
| Q9NS98 | SEMA3G | Semaphorin-3G | 0.425 | 0.161 | .001 |
| P02751 | FN1 | Fibronectin | 0.441 | 0.146 | .001 |
| P14543 | NID1 | Nidogen-1 | 0.442 | 0.190 | .006 |
| Q08431 | MFGE8 | Lactadherin | 0.446 | 0.167 | .026 |
| Q15848 | ADIPOQ | Adiponectin | 0.449 | 0.652 | .005 |
| O75390 | CS | Citrate synthase, mitochondrial | 0.454 | 0.183 | .012 |
| Q92626 | PXDN | Peroxidasin homolog | 0.457 | 0.200 | .001 |
| P07942 | LAMB1 | Laminin subunit β1 | 0.484 | 0.110 | .001 |
| Q08629 | SPOCK1 | Testican-1 | 0.497 | 0.055 | .014 |
| O00462 | MANBA | β-mannosidase | 2.180 | 2.560 | .034 |
| Q13510 | ASAH1 | Acid ceramidase | 2.222 | 3.135 | .048 |
| P02794 | FTH1 | Ferritin heavy chain | 2.451 | 0.769 | .030 |
| Q02952 | AKAP12 | A-kinase anchor protein 12 | 2.665 | 0.572 | .047 |
| A6NCN2 | KRT87P | Putative keratin-87 protein | 4.113 | 0.011 | .022 |
| P23468 | PTPRD | Receptor-type tyrosine-protein phosphatase δ | 10.687 | 2.199 | .003 |
| P10586 | PTPRF | Receptor-type tyrosine-protein phosphatase F | 11.935 | 3.955 | .001 |
NOTE. Proteins shown had a greater than 2-fold change in signal intensity in response to iron, with P < .05. Proteins highlighted in bold represent the 20 proteins of interest after review of the UniProt protein descriptions. Data were analyzed using the online Quantitative Proteomics P value Calculator using no normalization and nonadjusted P values (N = 3 per group).
CUB, C1r/C1s, Uegf, bone morphogenetic protein-1; EGF, epidermal growth factor; HTRA, High-Temperature Requirement A.