Figure 6.

Production of antioxidant secondary metabolites by T. purpureogenus. The fungus was grown in various culture media for different time period and organic extracts of the total fungal culture were tested for their potential antioxidative activity (IC₅₀ values) by DPPH radical scavenging assay. (A) Time course of DPPH scavenging of fungal crude extract of T. purpureogenus. The fungus was grown in PDB for indicated time period and the ethyl acetate extract was tested for antioxidative activity. (B) In vitro DPPH scavenging activity of different solvent extracts of T. purpureogenus. The fungus was grown in PDB for 21 days and different solvent extracts were tested for antioxidative activity. (C) Effect of different culture media on the production of antioxidative secondary metabolites by T. purpureogenus. The fungus was grown in different media for 21 days and the hexane extracts (TPHE) were tested for antioxidative activity. (D) Effect of NaCl on the production of antioxidant secondary metabolites by T. purpureogenus. The fungus was grown in MEB with different concentration of NaCl for 21 days and the hexane extracts (TPHE) were tested for antioxidative activity. Values are the means of three replicates ± SD of three replicates. Means sharing different alphabets “a,” “b” differ significantly from each other at p ≤ 0.05.