Fig. 5.

MTX induces alterations in various microbial taxa over time. (a) Heatmap of OTUs of intestinal microbiota in the MTX-treated mice (n = 7). (b, c) Examples of OTUs for which the abundance (b) decreased or (c) increased after MTX treatment. (d) Linear discriminant analysis (LDA) effect size (LEfSe) of the differentially abundant microbial taxa on day 0, day 7, and day 14 in the MTX-treated mice. Only taxa meeting an LDA significant threshold of >3.6 are shown. (e) Changes in Bacteroidales in mice treated with or without MTX. Shown are the means ±SEM; ns, not significant; * adjusted P < 0.05 and ** adjusted P < 0.01 versus day 0.