Figure 4.

Spontaneous colon cytokine production after chronic DSS-induced colitis. WT and ICE KO mice were fed with 2% DSS for 5 days followed by 5 days of regular water. This cycle was repeated three times, resulting in a 30-day experimental period. The colon was removed and washed extensively, and 1-cm² pieces were cultured for 24 h. Cytokines were measured in the supernatant, and protein concentration was determined as described in Materials and Methods. Bars are mean ± SEM. **, P < 0.01; ***, P < 0.001 vs. DSS WT.