Fig. 4.

Mapping the Asb2-interacting domains in IκBα. a Schematic diagram of HA-tagged Asb2 and its mutant constructs. The first and last Asb2 amino acids retained in the mutant constructs are denoted. b The Asb2 deletion constructs described in panel A were transfected into 293 cells. Immunoprecipitation was performed with anti-HA. The precipitates and inputs were probed with anti-HA and anti-IκBα