Fig. 3.

PvCSP(VK210) and PvCSP(VK247) expression in oocyst-derived sporozoites of two chimeric P. falciparum parasite lines (pf-pvcsp). Immunofluorescence analyses of wild type P. falciparum (WT) sporozoites and oocyst-derived pf-pvcsp sporozoites. Fixed sporozoites were labelled with mouse anti-PvCSP-VK210 mAb, anti-PvCSP-VK247mAb and mouse anti-PfCSP antibodies. As a control an antibody against PfHSP70 was used. Secondary conjugated antibodies used: anti-IgG Alexa Fluor^® 488 (green) or anti-IgG Alexa Fluor ^® 594 (red). Nuclei stained with the DNA-specific dye Hoechst-33342. All pictures were recorded with standardized exposure/gain times; Alexa Fluor^® 488 (green) 0.7 s; anti-IgG Alexa Fluor ^® 594 (red) 0.6 s; Hoechst (blue) 0.136 s; bright field 0.62 s (1× gain). Scale bar, 7 µm