Table 3.
Overview of the cfDNA extraction and variant analysis methods methods used by the participants
| # participants to KRAS analysis (%) (n = 23) | # participants to NRAS analysis (%) (n = 20) | # participants to EGFR analysis (%) (n = 31) | |
|---|---|---|---|
| cfDNA extraction method | |||
| QIAamp Circulating Nucleic AcidKit (Qiagen) | 14 (60.9) | 13 (65.0) | 17 (54.8) |
| Cobas cfDNA Sample Preparation Kit (Roche) | 4 (17.4) | 3 (15.0) | 8 (25.8) |
| MagMAX Cell-Free DNA Isolation Kit (Thermo Fisher Scientific) | 3 (13.0) | 3 (15.0) | 3 (9.7) |
| Maxwell® RSC ccfDNA Plasma Kit (Promega) | 1 (4.3) | 0 (0.0) | 1 (3.2) |
| Nucleospin Plasma XS (Macherey-Nagel) | 1 (4.3) | 1 (5.0) | 1 (3.2) |
| QIAamp DSP DNA Blood Mini Kit (Qiagen) version 2 | 0 (0.0) | 0 (0.0) | 1 (3.2) |
| Variant analysis method | |||
| NGS | 13 (56.5) | 13 (65.0) | 12 (38.7) |
| Commercial Kit | 4 (17.4) | 3 (15.0) | 11 (35.5) |
| LDT | 1 (4.3) | 0 (0.0) | 1 (3.2) |
| BEAMing | 1 (4.3) | 1 (5.0) | 0 (0.0) |
| ddPCR | 4 (17.4) | 3 (15.0) | 7 (22.6) |
The LDT consisted of a 5’nuclease polymerase-chain reaction (Taqman) with peptide nucleic acid probe. For a detailed breakdown of the used methods see Additional file 2: Table S2. Abbreviations: BEAMing Beads, emulsification, amplification, and magnetics, ddPCR Droplet digital polymerase chain reaction, LDT Labroratory-developed test, NGS Next-generation sequencing