Table 1. Pseudoperoxidase and peroxidase activities of the different Cyt-c variants a .
Activity | K m/mM–1 | k cat/s–1 | % of control | |
WT | Pseudoperoxidase | 144.3 c | 8 c | — |
Peroxidase | 65 d | 5.5 d | 100 | |
SO-Cyt ex situ | Pseudoperoxidase b | 0.24 (0.08) | 2.6 (0.8) | — |
Peroxidase | 19 e | — | 719 | |
SO-Cyt in situ | Pseudoperoxidase b | 0.44 f (0.07) | 1.8 f (0.6) | — |
Peroxidase | — | — | 644 i | |
M80A | Pseudoperoxidase | 0.00325 g | — | — |
Peroxidase | 23.2 h | 72 h | 780 |
aStandard deviations are indicated in brackets.
bDetermined for Cyt-c adsorbed on NH2-SAMs, this work.
cCyt-c adsorbed on COO-SAMs, from ref. 45.
dCyt-c in solution with ABTS, from ref. 63.
eSO-Cyt in solution with ABTS, from ref. 64.
fSO-Cyt obtained in situ on NH2-SAMs, this work.
gFrom ref. 60.
hIso-cyt M80A mutant, from ref. 65.
iSO-Cyt obtained by pretreatment with H2O2 in the presence of PC/PE liposomes.