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. 2014 Sep 1;6(1):705–713. doi: 10.1039/c4sc02181a

Table 1. Pseudoperoxidase and peroxidase activities of the different Cyt-c variants a .

Activity K m/mM–1 k cat/s–1 % of control
WT Pseudoperoxidase 144.3 c 8 c
Peroxidase 65 d 5.5 d 100
SO-Cyt ex situ Pseudoperoxidase b 0.24 (0.08) 2.6 (0.8)
Peroxidase 19 e 719
SO-Cyt in situ Pseudoperoxidase b 0.44 f (0.07) 1.8 f (0.6)
Peroxidase 644 i
M80A Pseudoperoxidase 0.00325 g
Peroxidase 23.2 h 72 h 780

aStandard deviations are indicated in brackets.

bDetermined for Cyt-c adsorbed on NH2-SAMs, this work.

cCyt-c adsorbed on COO-SAMs, from ref. 45.

dCyt-c in solution with ABTS, from ref. 63.

eSO-Cyt in solution with ABTS, from ref. 64.

fSO-Cyt obtained in situ on NH2-SAMs, this work.

gFrom ref. 60.

hIso-cyt M80A mutant, from ref. 65.

iSO-Cyt obtained by pretreatment with H2O2 in the presence of PC/PE liposomes.