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. 2018 Aug 10;8:30. doi: 10.1186/s13601-018-0216-9

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — A schematic representation of the sequential sampling during the combined gastric and duodenal digestion protocol. G stands for gastric, D for duodenal. The numbers indicate the time at which samples were taken in minutes. G0 and G0D0 samples have not been exposed to acidic pH. In total 9 different combinations of pH and pepsin-to-protein ratios were tested in the gastric phase. In combination with the duodenal phase per molecule a total of 144 samples (4 gastric plus 3 × 4 duodenal times 9 conditions) were analyzed by SDS-PAGE and immunoblotting