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DNA binding (KD) and lyase activity (kObs) of wild type Pol θ and various Lys mutants. *Double = K2575A/K2577A, **Triple = K2383A/K2575A/K2577A. KD values are the ave. ± std. dev. of 3 experiments utilizing different samples, and were determined using 6 via fluorescence anisotropy. kObs are the ave. ± std. dev. of at least 2 experiments determined using 3, each consisting of 3 replicates.
