. Author manuscript; available in PMC: 2019 Jul 25.

Published in final edited form as: J Am Chem Soc. 2018 Jul 12;140(29):9034–9037. doi: 10.1021/jacs.8b04158

Table 1.

Polymerase activity of wild type Pol θ and mutants.^a

Pol θ variant	K_m (μM)^b	k_cat (min⁻¹)^b	k_cat/K_m (μM•min) ⁻¹
wt²⁹	5 ± 1	65 ± 12	13.6
K2383A	1094 ± 37	0.5 ± 0.1	4.8 × 10⁻⁴
K2383R	311 ± 24	1.4 ± 0.2	4.3 × 10⁻³

Kinetics of dA incorporation in 5 were measured.

Data are the ave. ± std. dev. of 2 experiments each consisting of 3 replicates.