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Mobile-phase gradient for high-performance liquid chromatography analysis of amoxicillin samples
| Time (minutes) | Methanol (%) | 20 mM, Phosphate buffer pH = 4.4 ± 0.1 (%) | Change |
|---|---|---|---|
| 0.0 | 5.0 | 95.0 | Hold |
| 0.50 | 5.0 | 95.0 | Hold |
| 5.00 | 30.0 | 70.0 | Linear |
| 7.00 | 90.0 | 10.0 | Linear |
| 8.00 | 90.0 | 10.0 | Hold |
| 8.50 | 25.0 | 75.0 | Linear |
| 10.00 | 10.0 | 90.0 | Linear |
| 11.00 | 5.0 | 95.0 | Linear |
| 12.00 | 5.0 | 95.0 | Hold |
