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. 2018 Aug 1;29(15):1866–1877. doi: 10.1091/mbc.E18-03-0199

FIGURE 3:

FIGURE 3:

The Kip3 tail is not required for Kar9-dependent spindle positioning. (A) KIP3-Δdistal is not synthetic lethal with dyn1Δ. Dissection of tetrads resulting from a cross between KIP3-Δdistal and dyn1Δ haploid cells. Twenty-eight of 104 spores from 26 tetrads (∼1:4) possessed both KIP3-Δdistal and dyn1Δ mutations (yellow boxes). (B) Early mitotic spindles are properly positioned in cells harboring tailless Kip3ΔT-LZ or Kip3-Δdistal. Graph depicts position of the early mitotic spindle relative to the bud neck in small budded cells of the indicated genotype. For each category, kip3Δ is statistically different vs. each cell type. Graph shows three experiments where n = 239, 124, 127 for KIP3; 221, 111, 49 for KIP3-Δdistal; 182, 129, 150 for KIP3ΔT-LZ; 241, 140, 189 2x KIP3ΔT-LZ; and 180, 106, 117 for kip3Δ.