FIGURE 5:
Kar1 promotes premature SPB separation at prophase I. (A) Representative images showing GFP-Kar1 localization at prophase I. Projected images are shown. (B) Quantification of SPB separation in GFP-KAR1 ndt80Δ and PDMC1-GFP-KAR1 ndt80Δ cells. (C) Quantification of SPB separation in PCLB2-IPL1 ndt80Δ and PCLB2-IPL1 ndt80Δ PDMC1-KAR1 cells. (D) Quantification of SPB separation in PDMC1-GFP-KAR1 PGAL1-NDT80 cells. Arrows indicate the time of addition of EtOH and estradiol, which was dissolved in EtOH. (E) Quantification of SPB separation in PGAL1-GFP-KAR1 PGAL1-NDT80 cells. As in D, arrows indicate the time of addition of EtOH and estradiol. The time-course experiments were repeated, and data from one representative experiment are shown.