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. 2018 Aug 1;29(15):1798–1810. doi: 10.1091/mbc.E18-03-0163

FIGURE 6:

FIGURE 6:

Protein domains of Kar1 required for yeast meiosis. (A) Diagram showing the Kar1 protein structure. Deletions of kar1-Δ15 and -Δ18 have been described previously (Vallen et al., 1992). (B) Representative images showing the localization of GFP-Kar1-ΔTMD in yeast meiosis. Note that without its transmembrane domain (TMD), Kar1 fails to localize to the SPB, which is marked by Tub4-mApple. (C, D) Quantification of SPB separation in PDMC1-GFP-KAR1-ΔTMD cells. The ndt80Δ allele was used to arrest yeast cells at prophase I. (E, F) Quantification of SPB separation in PDMC1-GFP-KAR1-Δ15 cells. (G, H) Quantification of SPB separation in PDMC1-GFP-KAR1-Δ18 cells. At least 100 cells were counted at each time point. The time-course experiments were repeated, and data from one representative experiment are shown.