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. 2018 Aug 1;29(15):1798–1810. doi: 10.1091/mbc.E18-03-0163

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© 2018 Agarwal et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 7: — The execution point of Kar1-mediated SPB duplication in yeast meiosis. (A) Representative images showing GFP-Kar1 and Tub4 localization in P_DMC1-GFP-KAR1 P_CLB2-CDC20 cells. Depletion of Cdc20 arrests yeast cells at metaphase I. (B) Quantification of SPB separation in P_CLB2-CDC20 and P_DMC1-GFP-KAR1 P_CLB2-CDC20 cells. Note the formation of supernumerary SPBs in P_DMC1-GFP-KAR1 P_CLB2-CDC20 cells. (C) Representative images showing Sfi1 localization in P_DMC1-GFP-KAR1 cells at metaphase I. Note that Sfi1-GFP colocalizes with Tub4-mApple. (D) Representative images showing Mps3 localization in P_DMC1-GFP-KAR1 cells at metaphase I. (E) Representative images showing Tub1 localization in P_DMC1-GFP-KAR1 cells at metaphase I. Projected images are shown (C–E). (F) Quantification of SPB separation in Cdc5-depleted (P_CLB2-CDC5) cells in meiosis. (G) Quantification of SPB separation in P_DMC1-GFP-KAR1 P_CLB2-CDC5 cells. Representative images of GFP-Kar1 and Tub1-mApple are shown to the right. The time-course experiments were repeated, and data from one representative experiment are shown.