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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Clin Cancer Res. 2018 Mar 7;24(11):2616–2630. doi: 10.1158/1078-0432.CCR-17-1207

Figure 4. TAZ supports and is necessary for aRMS cancer cell stemness.

Figure 4.

Rh28 (left) and Rh30 (right) aRMS cells were grown as 3D-rhabdospheres. (A, C) Representative images of spheres. Scale bars, 100μm. (B, D) Compared to cells grown as an adherent monolayer, serial sphere passage increases TAZ expression, as measured by qRT-PCR. TAZ suppression (E, G) also leads to (F, H) decreased expression of stem cell genes SOX2, OCT4, and NANOG, as measured by qRT-PCR. mRNA expression was normalized to GAPDH. (I, J) Limiting dilution assays in cells stably expressing TAZ shRNAs show TAZ is necessary for stem cell renewal in aRMS cells. The average sphere-forming frequency is shown, with the expected range in parentheses. During LDA optimization, neither Rh28 nor Rh30 cells were able to form spheres when plated at 1 cell/well, and compared to Rh30 cells, Rh28 cells were less able to form spheres at lower cell densities. P=passage number.