FIGURE 5.

Early responses to Aβ42 exposure are oxidative stress and subtle nucleolar stress. (A) Flow Cytometry Experiment with CellROX Green showed that Aβ incubation for 2 h induces a significant level of oxidative stress. (B) Western blotting revealed that at the 2 h time point, Aβ causes a reduction of only UBF, not FBL or TIP5. Normalized to β-actin. Quantitative Click-iT RNA immunofluorescence labeling showed no change in newly synthesised RNA (C); H3K9me3 (D); or in newly synthesized proteins (E). N = 5, ^∗P < 0.05 (See also Supplementary Figure 4).