Fig. 4.

Cellulase-induced resistance in A. thaliana seedlings depends on the osmoticum during permeabilisation. Seedlings grown for 5–6 days in H₂O and pretreated ±cellulase in H₂O were transferred to different media. Fluorescence images were collected using PrI to detect permeabilisation by alamethicin (20 min, the last five with PrI probe present). Three hours Cellulase, 1%; Alamethicin, 20 μg ml^− 1; Mannitol, 0.35 M; Tricine, 50 mM, pH 8; MgCl_2, 3.0 mM; KCN 1.0 mM; nPG, n-propyl gallate 100 μM, isocitrate, 2 mM, NADP⁺ 1 mM. The images show one representative replicate out of three