Fig. 5.

CIRA detected in A. thaliana seedlings by NADP-ICDH assays. Seedlings were grown for 5 days as in Fig. 4. Data on NADP-ICDH activity was collected using a plate reader. a The effect of mannitol assayed with 13 μg ml^− 1 alamethicin. Mean values from four different experiments where n was 5–10 for each treatment within each experiment. Bars show SE. b The effect of alamethicin assayed in the presence of 100 mM mannitol. Average values ±SE for n = 6–11 seedling pools are shown. Final concentrations besides mannitol and alamethicin were as for Fig. 4. Significant differences ± cellulase are denoted with asterisks: ***, p < 0.001; **, p < 0.01