Table 1.

Summary of data to be obtained in the 3H study

Cardiovascular measures (annual intervals)	(i) High resolution sonography of the common carotid arteries to measure intima media thickness (morphology, B-Mode) and elasticity (function, M-mode)
	(ii) Pulse wave velocity and augmentation index
	(iii) Echocardiogram
	(iv) 24-h ambulatory blood pressure monitoring
Anthropometry
-6-monthly intervals-	(i) Weight, height, body mass index and pubertal staging
	(ii) Body composition analysis by multifrequency bioelectrical impedance analysis
Biomarker monitoring (6-monthly intervals)
-Nutritional measures	Albumin, prealbumin, leptin, ghrelin, cholecystokinin, endogenous growth hormone production (IGF-1, IGF-binding protein), adiponectin, resting energy expenditure (calculated), normalized protein catabolic rate (calculated), physical activity index
-Cardiovascular measures	calcium, phosphate, parathyroid hormone, FGF-23 (c-terminal), soluble klotho, 25-hydroxyvitamin D, 1,25-dihydroxyvitamin D, β2 microglobulin, fetuin-A, osteoprotegerin, markers of inflammation (IL6, IL-10, high-sensitivity CRP, TNF-α, plasma intradialytic endotoxin), markers of endothelial dysfunction (homocysteine, ADMA, SDMA), markers of bone turnover (bone-specific alkaline phosphatase, sclerostin, collagen telopeptides, β-cross-laps), markers of oxidative and carbonyl stress
Quality of life questionnaires (6-monthly intervals)	(i) Paediatric Index of Emotional Distress (Pi-ED)
	(ii) Paediatric Quality of Life (PedsQL)
	(iii) Strengths Difficulties Questionnaire (SDQ)
	(iv) Patient related outcome measures related to dialysis: post-dialysis recovery time, sleep pattern, school attendance, physical activity, appetite.