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. 2018 Aug 10;18:164. doi: 10.1186/s12870-018-1337-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Structure of embryonal masses from primary, secondary and tertiary lines of genotype SD4. a / SD4; b / SD4–2; c / SD4–8; d / SD4–8-1; e / SD4–8-2 f / SD4–8-3. Arrowheads in c, d mark small singulated somatic embryos; m – meristem of polyembryogenic centers, s – suspensor; Trypan blue staining of squashes of fresh EMs. Scale bar = 500 μm