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. Author manuscript; available in PMC: 2019 Aug 21.
Published in final edited form as: Neuroscience. 2018 Jun 27;386:214–222. doi: 10.1016/j.neuroscience.2018.06.029

Fig. 2. DAMGO-withdrawn planarians display abstinence-induced withdrawal responses. (A).

Fig. 2

Abstinence from DAMGO exposure enhances light avoidance (defensive responding). Planarians were exposed to different concentrations of DAMGO (0, 0.1, 1, 10 μM) for 30 min and then removed and placed into a petri dish containing water that was split evenly into light and dark sides. Time spent in the light was measured over 10 min. Data was presented as a scatter plot showing the median percentage of time spent in the light (with interquartile range) over the 10-min interval. N=11–12 planarians/group. *p < 0.05 compared to water control (0 μM). (B) Abstinence from DAMGO exposure decreases motility. Planarians were exposed to different concentrations of DAMGO (0, 0.1, 1, 10 μM) for 30 min and them removed and placed into a petri dish containing water. Motility counts were quantified as the number of gridlines that planarians crossed in 5 min. Data were presented as percentage of water control (+S.E.M.) over the 5-min interval. N=18 planarians/group. *p < 0.05 compared to water control (0 μM).