Figure 2 |. GLP1R-expressing GABAergic neurons in the MnPO are a major source of inhibitory input to the SFO.

a, GABAergic input to the SFO. Representative image of the SFO and MnPO after co-injection of AAV-Syn-GCaMP6s (green) and HSV-mCherry (red) in the SFO (one out of four mice). b, GLP1R expression is enriched in inhibitory neurons from the lamina terminalis (LT) relative to the cortex. c, MnPO^GLP1R neurons are GABAergic (84.7 ± 3.4% of GAD⁺ neurons are tdTomato⁺, n = 3 mice, representative images are from one out of three mice). These neurons did not overlap with glutamatergic neurons (4.3 ± 0.9% overlap, n = 3 mice, Extended Data Fig. 6a). d, The MnPO^GLP1R → SFO monosynaptic connection. MnPO^GLP1R neurons send monosynaptic inhibitory input to SFO^nNOS neurons. e, Optogenetic stimulation of MnPO^GLP1R neurons selectively suppresses water intake (n = 7 mice for ChR2 and n = 6 mice for control). ***P < 0.001, ****P < 0.0001, by paired two-tailed t-test. All error bars show mean ± s.e.m. Scale bars, 50 μm.