Table 2.
DENV serotypes and YFV isolates tested in side by side comparisons of the DENV–YFV assay and comparator rRT-PCRs for each virus
| Virus | Detected | ||
|---|---|---|---|
| Concentration (cp/μL)* | DENV–YFV† | Comparator† | |
| DENV serotype | |||
| DENV-1 | 60–355,700 | 12/12 | 12/12 |
| DENV-2 | 1,800–381,700 | 12/12 | 12/12 |
| DENV-3 | 400–186,300 | 24/24 | 24/24 |
| YFV isolate‡ | |||
| INHRR 10a-10–Venezuela 2010 | 10–10,800 | 5/5 | 5/5 |
| Couma–Ethiopia 1961 | 5–499,500 | 9/9 | 8/9 |
| MIS 1034–Peru 2011 | 6–5,800 | 5/5 | 4/5 |
| FMD 1240–Peru 2007 | 4–35,300 | 7/7 | 7/7 |
| FVB 0196–Bolivia 2006 | 2–23,100 | 7/7 | 6/7 |
| CAREC M2-09–Trinidad 2009 | 8–8,400 | 6/7 | 3/7 |
| SH 281586, Angola 2016, human serum | 4–299,800 | 9/9 | 8/9 |
| DakArA MT7, Cote d’Ivoire 1973 | 5–51,600 | 7/9 | 8/9 |
| ArD 114896, Senegal 1965 | 3–28,100 | 7/7 | 5/7 |
DENV = dengue virus; rRT-PCR = real-time reverse transcription PCR; YFV = yellow fever virus.
*
Concentration expressed as copies per microliter of eluate for comparison with the lower limit of 95% detection .
†
Number of replicates detected/number tested for each assay over the range of concentrations shown in the table.
‡
The listed concentrations include all dilutions that tested positive in one or both YFV assays. Replicates with negative results were the lowest concentration tested for each isolate; for CAREC M2-09, the YFV comparator tested negative with replicates at 8 and 80 cp/μL.